Virology and Drug Discovery Core
The objectives of the Virology and Drug Discovery Core are: a) to provide economy of scale through the provision of shared resources; b) to facilitate academic and industrial collaborative development of HIV vaccines and HIV prevention, therapeutic and diagnostic strategies; c) to promote virology & drug discovery education and training opportunities for CFAR investigators and local, national and international AIDS investigators; and d) to foster interdisciplinary collaboration among and between basic, clinical, and behavioral HIV/AIDS investigators locally, nationally and internationally.
| Lab Specific Information | ||
| Clinical Virology Lab | Pre-Clinical Virology Lab | Drug Discovery Lab |
CLINICAL VIROLOGY LAB
Angela Caliendo, MD, PhD
Professor, Department of Pathology
Emory Hospital Clinical Laboratory
Emory University School of Medicine
Phone: 404-712-5721
Email: acalien@emory.edu
For laboratory services, contact:
Jess Ingersoll
Laboratory Coordinator
Phone: 404-727-4483
E-mail: jessica.ingersoll@emoryhealthcare.org
PRE-CLINICAL VIROLOGY LAB
David Garber, PhD
Assistant Professor, Department of Medicine (Infectious Diseases)
Emory Vaccine Center Virology Laboratory
Emory University School of Medicine
Phone: 404-727-4374
Email: dgarber@rmy.emory.edu
For laboratory services, contact:
Natalia Kozyr, PhD
Research Associate
Phone: 404-727-3043
E-mail: nkozyr@rmy.emory.edu
DRUG DISCOVERY LAB
Raymond Schinazi, PhD, DSc
Professor and Director, Department of Pediatrics
Laboratory of Biochemical Pharmacology
Emory University School of Medicine
Phone: 404-728-7711
Email: rschina@emory.edu
For laboratory services, contact:
Dana Ray
Research Project Coordinator
Phone: 404-728-7711
Email: dmray@emory.edu
VIRAL QUANTIFICATION SERVICES
Emory (Caliendo Lab)
- HIV-1 RNA
- HCV RNA
- SHIV/SIV RNA
- HIV -2 RNA
- HIV 1 Proviral DNA
- YFV RNA
Emory (Garber Lab)
- SHIV/SIV mac239 - Gag region
- SIM sm - UTR region
- Proviral SIV DNA assay
- HIV-2 - UTR region
- YVF - NS5 region
- MVA - UDG region
VA (Schinazi Lab)
- HBV DNA
MOLECULAR TESTING SERVICES
Emory (Caliendo Lab)
- C. trachomatis
- N. gonorrhoeae
- T. vaginalis
RESISTANCE ASSAY SERVICES
Emory (Caliendo Lab)
- HIV-1 genotypic drug resistance testing
VA (Schinazi Lab)
- HIV Phenotypic drug resistance testing
- HIV, HBV and HCV genotyping
- Selection of drug-resistant virus in vitro
- Other phenotypic testing
- Site directed mutagenesis for HIV-1, HIV-2, HCV, HBV, & SIV
DRUG DISCOVERY SERVICES
VA (Schinazi Lab)
- Intracellular drug uptake and egress
- Quantification of drugs in cells, plasma, and other fluids
- Bone marrow cytotoxicity assays
- Mitochondrial toxicity and lactic acid assays
- Other cell based toxicological assays on request
- Pharmacokinetic and phamacodynamic analyses
- Ability to characterize unknown metabolites by LC-MS-MS
- Drug characterization and purification of compounds from natural product sources
- Mechanistic and biochemical studies including determination of affinity of compounds for various viral and cellular enzymes
- Other mechanistic studies on request (e.g., time of addition)
- Nucleoside triphosphate synthesis
- Cell based antiviral assays (HIV, HBV, HCV, herpes simplex viruses, and others on request)
FEES
Please inquire about fees specific to your request.
TRAINING
Training is available for the above assays and also for BL-2/3 practices. Please inquire for details, availability, and requirements.
CORE FACILITIES
The core has the following dedicated and fully equipped laboratories:
- Biosafety Level-2 and 3
- PCR/Molecular Diagnostics
- Drug Discovery